2X7J

Structure of the menaquinone biosynthesis protein MenD from Bacillus subtilis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	2-SUCCINYL-5-ENOLPYRUVYL-6-HYDROXY-3-CYCLOHEXENE -1-CARBOXYLATE SYNTHASE	polymer	604	66954.8	4	UniProt (P23970) Pfam (PF02776) Pfam (PF16582) Pfam (PF02775)	BACILLUS SUBTILIS	MENAQUINONE BIOSYNTHESIS PROTEIN MEND, SEPHCHC SYNTHASE, MEND
2	E, K, Q, W (A, B, C, D)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
3	F, L, R, X (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
4	G, M, S, Y (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	H, N, T, Z (A, B, C, D)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	AA, BA, I, J, O... (D, A, B, C)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
7	CA, DA, EA, FA (A, B, C, D)	water	water		18.0	1074	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 580 (UniProt: P23970)

PDB	External Database	Details
Met -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Leu -1	-	expression tag
Glu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	267819.3
Non-Polymers*	Number of molecules	24
	Total formula weight	3029.7
All*	Total formula weight	270849.1

*Water molecules are not included.