2X7J
Structure of the menaquinone biosynthesis protein MenD from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.180, 152.990, 158.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.140 - 2.350 |
| R-factor | 0.17522 |
| Rwork | 0.172 |
| R-free | 0.22803 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.840 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 101958 | |
| <I/σ(I)> | 17.3 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN BUFFER CONTAINED 20 MM TRIS, 50 MM NACL, PH 7.5. CRYSTALLISATION CONDITION CONTAINED 35% PEG 1.5K, 0.1 M TRIS PH 8, 0.25 M AMMONIUM SULFATE |
