2WVQ

Structure of the HET-s N-terminal domain. Mutant D23A, P33H

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	SMALL S PROTEIN	polymer	225	25457.0	2	UniProt (Q03689) Pfam (PF14479) In PDB	PODOSPORA ANSERINA	HET-S MUTANT D23A, P33H
2	B	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	1	Chemie (DTT)
3	B	(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL	non-polymer		154.3	1	Chemie (DTU)
4		water	water		18.0	198	Chemie (HOH)

Sequence modifications

A, B: 13 - 221 (UniProt: Q03689)

PDB	External Database	Details
Met -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
Leu 7	-	expression tag
Val 8	-	expression tag
Pro 9	-	expression tag
Arg 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Ala 23	Asp 23	engineered mutation
His 33	Pro 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50913.9
Non-Polymers*	Number of molecules	2
	Total formula weight	308.5
All*	Total formula weight	51222.4

*Water molecules are not included.