2WVQ
Structure of the HET-s N-terminal domain. Mutant D23A, P33H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.330, 83.150, 67.360 |
Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
Resolution | 65.230 - 2.000 |
R-factor | 0.22725 |
Rwork | 0.225 |
R-free | 0.26947 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wvn |
RMSD bond length | 0.016 |
RMSD bond angle | 1.739 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.460 |
Number of reflections | 34078 | |
<I/σ(I)> | 10.5 | 3.7 |
Completeness [%] | 95.2 | 93.3 |
Redundancy | 3.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 276 | CRYSTALLIZED AT 276K IN 25% ETHYLENE GLYCOL |