2WPT

The crystal structure of Im2 in complex with colicin E9 DNase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	COLICIN-E2 IMMUNITY PROTEIN	polymer	86	9953.0	1	UniProt (P04482) Pfam (PF01320) In PDB	ESCHERICHIA COLI	IMME2, MICROCIN-E2 IMMUNITY PROTEIN
2	B	COLICIN-E9	polymer	134	15166.1	1	UniProt (P09883) Pfam (PF21431) In PDB	ESCHERICHIA COLI	E9 DNASE
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A, B	NITRATE ION	non-polymer		62.0	3	Chemie (NO3)
5		water	water		18.0	111	Chemie (HOH)

Sequence modifications

A: 1 - 86 (UniProt: P04482)

PDB	External Database	Details
Ala 23	Cys 23	engineered mutation
Cys 31	Glu 31	engineered mutation

B: 1 - 1 (PDB: 2WPT)
B: 2 - 134 (UniProt: P09883)

PDB	External Database	Details
Cys 95	Gly 543	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25119.1
Non-Polymers*	Number of molecules	5
	Total formula weight	370.2
All*	Total formula weight	25489.3

*Water molecules are not included.