2WPT
The crystal structure of Im2 in complex with colicin E9 DNase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-06 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.229, 82.897, 89.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.590 - 1.780 |
R-factor | 0.19434 |
Rwork | 0.193 |
R-free | 0.22288 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fsj |
RMSD bond length | 0.020 |
RMSD bond angle | 1.726 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.140 | 0.440 |
Number of reflections | 26874 | |
<I/σ(I)> | 14.1 | 2.46 |
Completeness [%] | 97.6 | 78.6 |
Redundancy | 6.7 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | pH 6.8 |