2WNN

Structure of wild type E. coli N-acetylneuraminic acid lyase in complex with pyruvate in space group P21

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-ACETYLNEURAMINATE LYASE	polymer	303	33596.4	4	UniProt (P0A6L4) Pfam (PF00701)	ESCHERICHIA COLI	N-ACETYLNEURAMINIC ACID LYASE, N-ACETYLNEURAMINATE PYRUVATE-LYASE, SIALIC ACID LYASE, SIALATE LYASE, SIALIC ACID ALDOLASE, NALASE
2	E (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
3	F, G, H (B, C)	PENTAETHYLENE GLYCOL	non-polymer		238.3	3	Chemie (1PE)
4	I, J, K, L (A, B, C, D)	water	water		18.0	758	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 296 (UniProt: P0A6L4)

PDB	External Database	Details
Met -6	-	expression tag
Glu -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134385.4
Non-Polymers*	Number of molecules	4
	Total formula weight	737.8
All*	Total formula weight	135123.2

*Water molecules are not included.