2WNN

Structure of wild type E. coli N-acetylneuraminic acid lyase in complex with pyruvate in space group P21

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0008747	molecular_function	N-acetylneuraminate lyase activity
A	0016829	molecular_function	lyase activity
A	0019262	biological_process	N-acetylneuraminate catabolic process
A	0042802	molecular_function	identical protein binding
A	0044010	biological_process	single-species biofilm formation
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005975	biological_process	carbohydrate metabolic process
B	0008747	molecular_function	N-acetylneuraminate lyase activity
B	0016829	molecular_function	lyase activity
B	0019262	biological_process	N-acetylneuraminate catabolic process
B	0042802	molecular_function	identical protein binding
B	0044010	biological_process	single-species biofilm formation
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0008747	molecular_function	N-acetylneuraminate lyase activity
C	0016829	molecular_function	lyase activity
C	0019262	biological_process	N-acetylneuraminate catabolic process
C	0042802	molecular_function	identical protein binding
C	0044010	biological_process	single-species biofilm formation
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005975	biological_process	carbohydrate metabolic process
D	0008747	molecular_function	N-acetylneuraminate lyase activity
D	0016829	molecular_function	lyase activity
D	0019262	biological_process	N-acetylneuraminate catabolic process
D	0042802	molecular_function	identical protein binding
D	0044010	biological_process	single-species biofilm formation

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE 1PE B 1298

Chain	Residue
B	PRO140
B	ALA141
B	GLY144
B	LYS146
B	HOH2109
C	ALA141

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE 1PE B 1299

Chain	Residue
B	ASP170
B	GLN173
B	TYR190
B	GLU192
B	HOH2192
B	HOH2194
D	ASP170
B	PRO140
B	SER168
B	GLY169

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE 1PE C 1297

Chain	Residue
A	GLY169
A	GLU192
A	ILE193
C	GLY169
C	ASP170
C	LEU171
C	TYR172

---

Functional Information from PROSITE/UniProt

site_id	PS00665
Number of Residues	18
Details	DHDPS_1 Dihydrodipicolinate synthase signature 1. GLYvgGSTGEAfvqslsE

Chain	Residue	Details
A	GLY41-GLU58

---

site_id	PS00666
Number of Residues	31
Details	DHDPS_2 Dihydrodipicolinate synthase signature 2. YNIPalSgvkLtldqintlvtlpg.VgALKQT

Chain	Residue	Details
A	TYR137-THR167

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"24521460","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Schiff-base intermediate with substrate","evidences":[{"source":"PubMed","id":"12711733","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19923724","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24521460","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9047371","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8081752","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"9047371","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12711733","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1FDY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FDZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1HL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24521460","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BWL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Site: {"description":"Required to correctly position the proton donor","evidences":[{"source":"PubMed","id":"24521460","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 553

Chain	Residue	Details
A	TYR137	proton acceptor, proton donor, proton relay
A	KPI165	covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor

---

site_id	MCSA2
Number of Residues	2
Details	M-CSA 553

Chain	Residue	Details
B	TYR137	proton acceptor, proton donor, proton relay
B	KPI165	covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor

---

site_id	MCSA3
Number of Residues	2
Details	M-CSA 553

Chain	Residue	Details
C	TYR137	proton acceptor, proton donor, proton relay
C	KPI165	covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor

---

site_id	MCSA4
Number of Residues	2
Details	M-CSA 553

Chain	Residue	Details
D	TYR137	proton acceptor, proton donor, proton relay
D	KPI165	covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor

---