2WNH

Crystal Structure Analysis of Klebsiella sp ASR1 Phytase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	3-PHYTASE	polymer	418	46115.3	2	UniProt (Q84CN9) Pfam (PF00328)	KLEBSIELLA PNEUMONIAE
2	C, F, G (A, B)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
3	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	E, H (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	I, J (A, B)	water	water		18.0	771	Chemie (HOH)

Sequence modifications

A, B: 13 - 405 (UniProt: Q84CN9)

PDB	External Database	Details
Met 1	-	expression tag
Asp 2	-	expression tag
Ile 3	-	expression tag
Gly 4	-	expression tag
Ile 5	-	expression tag
Asn 6	-	expression tag
Ser 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Pro 10	-	expression tag
Pro 11	-	expression tag
Arg 12	-	expression tag
Ala 123	Val 139	engineered mutation
Ser 279	Asn 295	engineered mutation
Ala 397	Thr 413	engineered mutation
Lys 406	-	expression tag
Leu 407	-	expression tag
Ala 408	-	expression tag
Ala 409	-	expression tag
Ala 410	-	expression tag
Leu 411	-	expression tag
Glu 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
Ser 279	Asn 295	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92230.6
Non-Polymers*	Number of molecules	6
	Total formula weight	277.5
All*	Total formula weight	92508.1

*Water molecules are not included.