2WKQ

Structure of a photoactivatable Rac1 containing the Lov2 C450A Mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NPH1-1, RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 1	polymer	332	37570.9	1	UniProt (O49003) UniProt (P63000) Pfam (PF13426) Pfam (PF00071) In PDB	AVENA SATIVA (OAT)	P21-RAC1, RAS-LIKE PROTEIN TC25, CELL MIGRATION-INDUCING GENE 5 PROTEIN
2	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
3	A	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
6	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7		water	water		18.0	397	Chemie (HOH)

Sequence modifications

A: 404 - 546 (UniProt: O49003)

PDB	External Database	Details
Ala 450	Cys 450	engineered mutation

A: 547 - 723 (UniProt: P63000)

PDB	External Database	Details
Met 392	-	expression tag
Arg 393	-	expression tag
Gly 394	-	expression tag
Ser 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
Gly 402	-	expression tag
Ser 403	-	expression tag
Leu 604	Gln 61	engineered mutation
His 634	Glu 91	engineered mutation
His 635	Asn 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37570.9
Non-Polymers*	Number of molecules	8
	Total formula weight	1234.3
All*	Total formula weight	38805.2

*Water molecules are not included.