2WKQ

Structure of a photoactivatable Rac1 containing the Lov2 C450A Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000242	cellular_component	pericentriolar material
A	0001764	biological_process	neuron migration
A	0001891	cellular_component	phagocytic cup
A	0001934	biological_process	positive regulation of protein phosphorylation
A	0003376	biological_process	sphingosine-1-phosphate receptor signaling pathway
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005802	cellular_component	trans-Golgi network
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005884	cellular_component	actin filament
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0005938	cellular_component	cell cortex
A	0006911	biological_process	phagocytosis, engulfment
A	0006954	biological_process	inflammatory response
A	0007015	biological_process	actin filament organization
A	0007155	biological_process	cell adhesion
A	0007160	biological_process	cell-matrix adhesion
A	0007163	biological_process	establishment or maintenance of cell polarity
A	0007167	biological_process	enzyme-linked receptor protein signaling pathway
A	0007264	biological_process	small GTPase-mediated signal transduction
A	0008045	biological_process	motor neuron axon guidance
A	0008360	biological_process	regulation of cell shape
A	0009611	biological_process	response to wounding
A	0009653	biological_process	anatomical structure morphogenesis
A	0010310	biological_process	regulation of hydrogen peroxide metabolic process
A	0010591	biological_process	regulation of lamellipodium assembly
A	0010592	biological_process	positive regulation of lamellipodium assembly
A	0010595	biological_process	positive regulation of endothelial cell migration
A	0010762	biological_process	regulation of fibroblast migration
A	0010764	biological_process	negative regulation of fibroblast migration
A	0010810	biological_process	regulation of cell-substrate adhesion
A	0016020	cellular_component	membrane
A	0016477	biological_process	cell migration
A	0016601	biological_process	Rac protein signal transduction
A	0016787	molecular_function	hydrolase activity
A	0019899	molecular_function	enzyme binding
A	0019901	molecular_function	protein kinase binding
A	0030027	cellular_component	lamellipodium
A	0030031	biological_process	cell projection assembly
A	0030032	biological_process	lamellipodium assembly
A	0030036	biological_process	actin cytoskeleton organization
A	0030041	biological_process	actin filament polymerization
A	0030334	biological_process	regulation of cell migration
A	0030425	cellular_component	dendrite
A	0030667	cellular_component	secretory granule membrane
A	0030672	cellular_component	synaptic vesicle membrane
A	0030742	molecular_function	GTP-dependent protein binding
A	0030865	biological_process	cortical cytoskeleton organization
A	0030900	biological_process	forebrain development
A	0031410	cellular_component	cytoplasmic vesicle
A	0031529	biological_process	ruffle organization
A	0031901	cellular_component	early endosome membrane
A	0031996	molecular_function	thioesterase binding
A	0032496	biological_process	response to lipopolysaccharide
A	0032587	cellular_component	ruffle membrane
A	0032707	biological_process	negative regulation of interleukin-23 production
A	0032956	biological_process	regulation of actin cytoskeleton organization
A	0034446	biological_process	substrate adhesion-dependent cell spreading
A	0035025	biological_process	positive regulation of Rho protein signal transduction
A	0035556	biological_process	intracellular signal transduction
A	0035567	biological_process	non-canonical Wnt signaling pathway
A	0035774	biological_process	positive regulation of insulin secretion involved in cellular response to glucose stimulus
A	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
A	0042470	cellular_component	melanosome
A	0042554	biological_process	superoxide anion generation
A	0042734	cellular_component	presynaptic membrane
A	0042995	cellular_component	cell projection
A	0043020	cellular_component	NADPH oxidase complex
A	0043652	biological_process	engulfment of apoptotic cell
A	0044877	molecular_function	protein-containing complex binding
A	0045202	cellular_component	synapse
A	0045211	cellular_component	postsynaptic membrane
A	0045428	biological_process	regulation of nitric oxide biosynthetic process
A	0045730	biological_process	respiratory burst
A	0046425	biological_process	regulation of receptor signaling pathway via JAK-STAT
A	0048012	biological_process	hepatocyte growth factor receptor signaling pathway
A	0048261	biological_process	negative regulation of receptor-mediated endocytosis
A	0048812	biological_process	neuron projection morphogenesis
A	0048870	biological_process	cell motility
A	0051022	molecular_function	Rho GDP-dissociation inhibitor binding
A	0051492	biological_process	regulation of stress fiber assembly
A	0051496	biological_process	positive regulation of stress fiber assembly
A	0051668	biological_process	localization within membrane
A	0051894	biological_process	positive regulation of focal adhesion assembly
A	0055038	cellular_component	recycling endosome membrane
A	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
A	0060091	cellular_component	kinocilium
A	0060263	biological_process	regulation of respiratory burst
A	0060326	biological_process	cell chemotaxis
A	0061344	biological_process	regulation of cell adhesion involved in heart morphogenesis
A	0070062	cellular_component	extracellular exosome
A	0071526	biological_process	semaphorin-plexin signaling pathway
A	0090023	biological_process	positive regulation of neutrophil chemotaxis
A	0097178	biological_process	ruffle assembly
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0101003	cellular_component	ficolin-1-rich granule membrane
A	0150052	biological_process	regulation of postsynapse assembly
A	1900026	biological_process	positive regulation of substrate adhesion-dependent cell spreading
A	1900029	biological_process	positive regulation of ruffle assembly
A	1900242	biological_process	regulation of synaptic vesicle endocytosis
A	1902622	biological_process	regulation of neutrophil migration
A	1903348	biological_process	positive regulation of bicellular tight junction assembly
A	1904948	biological_process	midbrain dopaminergic neuron differentiation
A	2000386	biological_process	positive regulation of ovarian follicle development

Functional Information from PDB Data

site_id	AC1
Number of Residues	26
Details	BINDING SITE FOR RESIDUE GTP A 1722

Chain	Residue
A	GLY555
A	PRO577
A	THR578
A	GLY603
A	LYS659
A	ASP661
A	LEU662
A	SER701
A	ALA702
A	LEU703
A	MG1724
A	ALA556
A	HOH2164
A	HOH2225
A	HOH2388
A	HOH2389
A	HOH2390
A	HOH2391
A	HOH2392
A	VAL557
A	GLY558
A	LYS559
A	THR560
A	CYS561
A	PHE571
A	TYR575

---

site_id	AC2
Number of Residues	30
Details	BINDING SITE FOR RESIDUE FMN A 1723

Chain	Residue
A	VAL416
A	THR418
A	ASN425
A	ASN449
A	ALA450
A	ARG451
A	LEU453
A	GLN454
A	VAL463
A	ARG464
A	ILE466
A	ARG467
A	ARG467
A	ILE470
A	LEU480
A	ASN482
A	ASN492
A	PHE494
A	LEU496
A	PHE509
A	ILE510
A	GLY511
A	GLN513
A	CL1726
A	HOH2020
A	HOH2050
A	HOH2393
A	HOH2394
A	HOH2395
A	HOH2396

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 1724

Chain	Residue
A	THR560
A	THR578
A	GTP1722
A	HOH2225
A	HOH2391

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 1725

Chain	Residue
A	SER626
A	LYS659
A	HOH2258

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL A 1726

Chain	Residue
A	ASN425
A	ASN449
A	ARG467
A	ILE470
A	FMN1723

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 1727

Chain	Residue
A	THR704
A	ARG706
A	HOH2280

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1728

Chain	Residue
A	GLY516
A	THR517
A	GLU699
A	HOH2107
A	HOH2365
A	HOH2397

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 1729

Chain	Residue
A	THR477
A	GLN479
A	LEU493
A	GLU525
A	HOH2120

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI7
Number of Residues	8
Details	Motif: {"description":"Effector region","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8WEJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8WEJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	Modified residue: {"description":"(Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate","evidences":[{"source":"PubMed","id":"19362538","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"(Microbial infection) O-AMP-threonine; by Vibrio VopS","evidences":[{"source":"PubMed","id":"19039103","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	1
Details	Glycosylation: {"description":"(Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB, and by P.sordellii toxin TcsL; alternate","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24905543","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7775453","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7777059","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"18093184","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"23512198","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	LEU604

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	ALA556

---