2WK3

Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-42)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	INSULIN DEGRADING ENZYME	polymer	1019	117968.7	2	UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187)	HOMO SAPIENS (HUMAN)	INSULIN PROTEASE, INSULYSIN, INSULINASE
2	C, D (C, D)	BETA-AMYLOID PROTEIN 42	polymer	42	4520.1	2	UniProt (P05067) Pfam (PF03494)	HOMO SAPIENS (HUMAN)	BETA-APP42, AMYLOID BETA A4 PROTEIN SYNONYM: AMYLOID BETA, ALZHEIMER DISEASE AMYLOID PROTEIN, ABPP, APPI, APP, PREA4, CEREBRAL VASCULAR AMYLOID PEPTIDE, CVAP, PROTEASE NEXIN-II, PN-II SYNONYM: APP, ABPP, ALZHEIMER'S DISEASE AMYLOID PROTEIN, CEREBRAL VASCULAR AMYLOID PEPTIDE, CVAP, PROTEASE NEXIN-II, PN-II, APPI
3	E, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	G, H (A, B)	water	water		18.0	234	Chemie (HOH)

Sequence modifications

A, B: 1 - 1019 (UniProt: P14735)

PDB	External Database	Details
Leu 110	Cys 110	engineered mutation
Gln 111	Glu 111	engineered mutation
Ser 171	Cys 171	engineered mutation
Ala 178	Cys 178	engineered mutation
Val 257	Cys 257	engineered mutation
Leu 414	Cys 414	engineered mutation
Asn 573	Cys 573	engineered mutation
Ser 590	Cys 590	engineered mutation
Ser 789	Cys 789	engineered mutation
Ala 812	Cys 812	engineered mutation
Ala 819	Cys 819	engineered mutation
Ser 904	Cys 904	engineered mutation
Asn 966	Cys 966	engineered mutation
Ala 974	Cys 974	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	244977.5
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	245108.3

*Water molecules are not included.