2W22

Activation Mechanism of Bacterial Thermoalkalophilic Lipases

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	TRIACYLGLYCEROL LIPASE	polymer	389	43368.4	1	UniProt (Q59260)	GEOBACILLUS THERMOCATENULATUS
2	B (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D, E (A)	2-(2-{2-[2-(2-{2-[2-(2-{2-[4-(1,1,3,3-TETRAMETHYL-BUTYL)-PHENOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOX Y}-ETHOXY)-ETHANOL	non-polymer		602.8	2	Chemie (EGC)
5	F, G, H (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	3	Chemie (MPD)
6	I (A)	water	water		18.0	531	Chemie (HOH)

Sequence modifications

A: 1 - 1 (PDB: 2W22)
A: 2 - 389 (UniProt: Q59260)

PDB	External Database	Details
Tyr 355	Cys 383	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43368.4
Non-Polymers*	Number of molecules	7
	Total formula weight	1665.6
All*	Total formula weight	45034.0

*Water molecules are not included.