2VZX

Structural and spectroscopic characterization of photoconverting fluorescent protein Dendra2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Green fluorescent protein	polymer	231	26485.0	8	UniProt (Q8T6U0) Pfam (PF01353)	Dendronephthya sp. SSAL-2002
2	I, L (A, D)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
3	J, K (B, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	1280	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 225 (UniProt: Q8T6U0)

PDB	External Database	Details
Ala 40	Leu 40	engineered mutation
Val 61	Leu 61	engineered mutation
5sq 64	His 62	chromophore
5sq 64	Tyr 63	chromophore
5sq 64	Gly 64	chromophore
Phe 95	Tyr 95	engineered mutation
Lys 121	Asn 121	engineered mutation
Thr 123	Met 123	engineered mutation
Ala 188	Tyr 188	engineered mutation
Gly 199	Ser 199	engineered mutation
Ala 213	Gly 213	engineered mutation
Val 224	Ala 224	engineered mutation
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	211880.4
Non-Polymers*	Number of molecules	4
	Total formula weight	572.6
All*	Total formula weight	212453.0

*Water molecules are not included.