2VZX
Structural and spectroscopic characterization of photoconverting fluorescent protein Dendra2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.260, 76.850, 92.500 |
| Unit cell angles | 90.05, 108.17, 106.58 |
Refinement procedure
| Resolution | 33.500 - 2.000 |
| R-factor | 0.21517 |
| Rwork | 0.212 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zux |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.170 |
| Number of reflections | 113215 | |
| <I/σ(I)> | 12.15 | 4.42 |
| Completeness [%] | 94.1 | 94.5 |
| Redundancy | 1.95 | 1.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 32% PEG 3350, 0.1M MAGNESIUM NITRATE, PH 8.8 |
