2VTF

X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ENDO-BETA-N-ACETYLGLUCOSAMINIDASE	polymer	626	70082.4	2	UniProt (Q9ZB22) Pfam (PF03644) Pfam (PF21910) In PDB	ARTHROBACTER PROTOPHORMIAE
2	A, B	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	4	Chemie (B3P)
3	A, B	TRIETHYLENE GLYCOL	non-polymer		150.2	4	Chemie (PGE)
4		water	water		18.0	1500	Chemie (HOH)

Sequence modifications

A, B: -4 - 0 (PDB: 2VTF)
A, B: 1 - 621 (UniProt: Q9ZB22)

PDB	External Database	Details
Asp 43	Asn 67	engineered mutation
Thr 51	Lys 75	engineered mutation
Gln 173	Glu 197	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	140164.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1730.0
All*	Total formula weight	141894.9

*Water molecules are not included.