2VEI
Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | GLYCOSOMAL TRIOSEPHOSPHATE ISOMERASE | polymer | 238 | 25687.3 | 3 | UniProt (P04789) Pfam (PF00121) In PDB | TRYPANOSOMA BRUCEI BRUCEI | TRIOSEPHOSPHATE ISOMERASE, TIM, TRIOSE-PHOSPHATE ISOMERASE |
2 | A, B, C | SULFATE ION | non-polymer | 96.1 | 16 | Chemie (SO4) | |||
3 | water | water | 18.0 | 776 | Chemie (HOH) |
Sequence modifications
A, B, C: 15 - 72 (UniProt: P04789)
A, B, C: 80 - 234 (UniProt: P04789)
PDB | External Database | Details |
---|---|---|
Ser 15 | Asn 15 | conflict |
Pro 18 | Gln 18 | conflict |
Asp 19 | Gln 19 | conflict |
Gly 68 | Ile 68 | conflict |
Asn 69 | Ala 69 | conflict |
Ala 70 | Lys 70 | conflict |
Asp 71 | Ser 71 | conflict |
Ala 72 | Gly 72 | conflict |
PDB | External Database | Details |
---|---|---|
Ala 81 | Pro 81 | conflict |
Ser 82 | Ile 82 | conflict |
Trp 100 | Ala 100 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 77062.0 | |
Non-Polymers* | Number of molecules | 16 |
Total formula weight | 1537.0 | |
All* | Total formula weight | 78599.0 |