2V1Z

Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	BETA-LACTAMASE TEM	polymer	291	32447.0	1	UniProt (P62593) Pfam (PF00144) Pfam (PF13354) In PDB	ESCHERICHIA COLI	TEM-1, TEM-2, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2, TEM-16/CAZ-7, TEM-24/CAZ-6, IRT-4, PENICILLINASE ALLOSTERIC BETA-LACTAMASE INSERTANT
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3		water	water		18.0	352	Chemie (HOH)

Sequence modifications

A: 1 - 14 (UniProt: P62593)

PDB	External Database	Details
Cys 15	-	insertion
Arg 16	-	insertion
Thr 17	-	insertion
Ser 18	-	insertion
His 19	-	insertion
Arg 20	-	insertion
Pro 21	-	insertion
Cys 22	-	insertion
Gly 269	-	expression tag
Leu 270	-	expression tag
Glu 271	-	expression tag
Gln 272	-	expression tag
Lys 273	-	expression tag
Leu 274	-	expression tag
Ile 275	-	expression tag
Ser 276	-	expression tag
Glu 277	-	expression tag
Glu 278	-	expression tag
Asp 279	-	expression tag
Leu 280	-	expression tag
Asn 281	-	expression tag
Ser 282	-	expression tag
Ala 283	-	expression tag
Val 284	-	expression tag
Asp 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag
His 290	-	expression tag
His 291	-	expression tag

A: 23 - 268 (UniProt: P62593)

PDB	External Database	Details
Ile 64	Val 82	engineered mutation
Lys 84	Glu 102	engineered mutation
Val 164	Ala 182	engineered mutation
Arg 219	Glu 237	engineered mutation
Arg 247	Gln 265	engineered mutation
Lys 248	Ala 266	engineered mutation
Lys 249	Thr 267	engineered mutation
Thr 250	Met 268	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32447.0
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	32512.5

*Water molecules are not included.