2V1Z
Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-11 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.113, 70.609, 78.313 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.410 - 1.600 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1btl |
RMSD bond length | 0.018 |
RMSD bond angle | 1.672 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0031) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.040 | 0.220 |
Number of reflections | 34091 | |
<I/σ(I)> | 23.9 | 8.2 |
Completeness [%] | 96.7 | 93.2 |
Redundancy | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | RESERVOIR: BIS-TRIS 0.1M PH6.2, PEG6000 25%(W/V), NACL 0.3M, NAN3 0.02%(W/V). HANGING DROP: 1UL PROTEIN AND 1 UL RESERVOIR |