2UU9

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a Valine-ASL with cmo5U in position 34 bound to an mRNA with a GUG-codon in the A-site and paromomycin.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	16S RRNA	polymer	1522	493958.3	1		THERMUS THERMOPHILUS
2	B	30S RIBOSOMAL PROTEIN S2	polymer	256	29317.7	1	UniProt (P80371) Pfam (PF00318) In PDB	THERMUS THERMOPHILUS
3	C	30S RIBOSOMAL PROTEIN S3	polymer	239	26751.1	1	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	THERMUS THERMOPHILUS
4	D	30S RIBOSOMAL PROTEIN S4	polymer	209	24373.4	1	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	THERMUS THERMOPHILUS
5	E	30S RIBOSOMAL PROTEIN S5	polymer	162	17583.4	1	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	THERMUS THERMOPHILUS
6	F	30S RIBOSOMAL PROTEIN S6	polymer	101	11988.8	1	UniProt (Q5SLP8) Pfam (PF01250) In PDB	THERMUS THERMOPHILUS	TS9
7	G	30S RIBOSOMAL PROTEIN S7	polymer	156	18051.0	1	UniProt (P17291) Pfam (PF00177) In PDB	THERMUS THERMOPHILUS
8	H	30S RIBOSOMAL PROTEIN S8	polymer	138	15868.6	1	UniProt (Q5SHQ2) Pfam (PF00410) UniProt (by SIFTS) (P0DOY9) In PDB	THERMUS THERMOPHILUS
9	I	30S RIBOSOMAL PROTEIN S9	polymer	128	14410.6	1	UniProt (P80374) Pfam (PF00380) In PDB	THERMUS THERMOPHILUS
10	J	30S RIBOSOMAL PROTEIN S10	polymer	105	11955.0	1	UniProt (Q5SHN7) Pfam (PF00338) In PDB	THERMUS THERMOPHILUS
11	K	30S RIBOSOMAL PROTEIN S11	polymer	129	13737.9	1	UniProt (P80376) Pfam (PF00411) In PDB	THERMUS THERMOPHILUS
12	L	30S RIBOSOMAL PROTEIN S12	polymer	135	14920.8	1	UniProt (Q5SHN3) Pfam (PF00164) In PDB	THERMUS THERMOPHILUS
13	M	30S RIBOSOMAL PROTEIN S13	polymer	126	14338.9	1	UniProt (P80377) Pfam (PF00416) In PDB	THERMUS THERMOPHILUS
14	N	30S RIBOSOMAL PROTEIN S14	polymer	61	7158.7	1	UniProt (Q5SHQ1) Pfam (PF00253) UniProt (by SIFTS) (P0DOY6) In PDB	THERMUS THERMOPHILUS
15	O	30S RIBOSOMAL PROTEIN S15	polymer	89	10578.4	1	UniProt (Q5SJ76) Pfam (PF00312) In PDB	THERMUS THERMOPHILUS
16	P	30S RIBOSOMAL PROTEIN S16	polymer	88	10410.0	1	UniProt (Q5SJH3) Pfam (PF00886) In PDB	THERMUS THERMOPHILUS
17	Q	30S RIBOSOMAL PROTEIN S17	polymer	105	12325.7	1	UniProt (Q5SHP7) Pfam (PF00366) UniProt (by SIFTS) (P0DOY7) In PDB	THERMUS THERMOPHILUS
18	R	30S RIBOSOMAL PROTEIN S18	polymer	88	10258.3	1	UniProt (Q5SLQ0) Pfam (PF01084) In PDB	THERMUS THERMOPHILUS
19	S	30S RIBOSOMAL PROTEIN S19	polymer	93	10605.5	1	UniProt (Q5SHP2) Pfam (PF00203) In PDB	THERMUS THERMOPHILUS
20	T	30S RIBOSOMAL PROTEIN S20	polymer	106	11736.1	1	UniProt (P80380) Pfam (PF01649) In PDB	THERMUS THERMOPHILUS
21	U	30S RIBOSOMAL PROTEIN THX	polymer	27	3350.0	1	UniProt (Q5SIH3) Pfam (PF17070) In PDB	THERMUS THERMOPHILUS
22	X	RNA	polymer	5	1610.0	1		THERMUS THERMOPHILUS
23	Y	RNA	polymer	17	5490.3	1		THERMUS THERMOPHILUS
24	A	PAROMOMYCIN	non-polymer		615.6	1	Chemie (PAR)
25	A, B, E, N, Q	MAGNESIUM ION	non-polymer		24.3	161	Chemie (MG)
26	A, E, R	POTASSIUM ION	non-polymer		39.1	36	Chemie (K)
27	D, N	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

B: 1 - 1 (PDB: 2UU9)
B: 2 - 256 (UniProt: P80371)
C: 1 - 1 (PDB: 2UU9)
C: 2 - 239 (UniProt: P80372)
D: 1 - 1 (PDB: 2UU9)
D: 2 - 209 (UniProt: P80373)
E: 1 - 1 (PDB: 2UU9)
E: 2 - 162 (UniProt: Q5SHQ5)
G: 1 - 1 (PDB: 2UU9)
G: 2 - 156 (UniProt: P17291)
J: 1 - 1 (PDB: 2UU9)
J: 2 - 105 (UniProt: Q5SHN7)
K: 1 - 1 (PDB: 2UU9)
K: 2 - 129 (UniProt: P80376)
L: 1 - 4 (PDB: 2UU9)
L: 5 - 135 (UniProt: Q5SHN3)
M: 1 - 1 (PDB: 2UU9)
M: 2 - 126 (UniProt: P80377)
N: 1 - 1 (PDB: 2UU9)
N: 2 - 61 (UniProt: Q5SHQ1)
O: 1 - 1 (PDB: 2UU9)
O: 2 - 89 (UniProt: Q5SJ76)
Q: 1 - 1 (PDB: 2UU9)
Q: 2 - 105 (UniProt: Q5SHP7)
R: 1 - 1 (PDB: 2UU9)
R: 2 - 88 (UniProt: Q5SLQ0)
S: 1 - 1 (PDB: 2UU9)
S: 2 - 93 (UniProt: Q5SHP2)
T: 1 - 1 (PDB: 2UU9)
T: 2 - 106 (UniProt: P80380)
U: 1 - 1 (PDB: 2UU9)
U: 2 - 27 (UniProt: Q5SIH3)

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	790778.4
Non-Polymers*	Number of molecules	200
	Total formula weight	6067.1
All*	Total formula weight	796845.4

*Water molecules are not included.