2RHS
PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Phenylalanyl-tRNA synthetase alpha chain | polymer | 294 | 33395.8 | 2 | UniProt (Q4L5E3) Pfam (PF01409) | Staphylococcus haemolyticus | Phenylalanine--tRNA ligase alpha chain, PheRS |
| 2 | B, D (B, D) | Phenylalanyl-tRNA synthetase beta chain | polymer | 800 | 88753.9 | 2 | UniProt (Q4L5E4) Pfam (PF01588) Pfam (PF03483) Pfam (PF03484) Pfam (PF17759) Pfam (PF03147) | Staphylococcus haemolyticus | Phenylalanine--tRNA ligase beta chain, PheRS |
| 3 | E, H (A, C) | 1-{3-[(4-pyridin-2-ylpiperazin-1-yl)sulfonyl]phenyl}-3-(1,3-thiazol-2-yl)urea | non-polymer | 444.5 | 2 | Chemie (GAX) | |||
| 4 | F, I (B, D) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | G (C) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | J, K, L, M (A, B, C, D) | water | water | 18.0 | 946 | Chemie (HOH) |
Sequence modifications
A, C: 84 - 351 (UniProt: Q4L5E3)
B, D: 1 - 800 (UniProt: Q4L5E4)
| PDB | External Database | Details |
|---|---|---|
| Met 58 | - | expression tag |
| Gly 59 | - | expression tag |
| Ser 60 | - | expression tag |
| Ser 61 | - | expression tag |
| His 62 | - | expression tag |
| His 63 | - | expression tag |
| His 64 | - | expression tag |
| His 65 | - | expression tag |
| His 66 | - | expression tag |
| His 67 | - | expression tag |
| Ser 68 | - | expression tag |
| Ser 69 | - | expression tag |
| Gly 70 | - | expression tag |
| Leu 71 | - | expression tag |
| Val 72 | - | expression tag |
| Pro 73 | - | expression tag |
| Arg 74 | - | expression tag |
| Gly 75 | - | expression tag |
| Ser 76 | - | expression tag |
| His 77 | - | expression tag |
| Met 78 | - | expression tag |
| Gly 79 | - | expression tag |
| Thr 80 | - | expression tag |
| Glu 81 | - | expression tag |
| Leu 82 | - | expression tag |
| Met 83 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 34 | Asp 34 | engineered mutation |
| Pro 144 | Gln 144 | engineered mutation |
| Gly 661 | Glu 661 | engineered mutation |
| Ala 663 | Gln 663 | engineered mutation |
| Gly 664 | Asp 664 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 244299.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1146.6 | |
| All* | Total formula weight | 245446.0 |
