2RGN

Crystal Structure of p63RhoGEF complex with Galpha-q and RhoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha	polymer	353	41298.9	2	UniProt (P10824) Pfam (PF00503) UniProt (by SIFTS) (P21279)	Rattus norvegicus (Rat)	Adenylate cyclase-inhibiting G alpha protein,Guanine nucleotide-binding protein alpha-q
2	B, E (B, E)	Rho guanine nucleotide exchange factor 25	polymer	354	40605.4	2	UniProt (Q86VW2) Pfam (PF00621) Pfam (PF22697)	Homo sapiens (Human)	Guanine nucleotide exchange factor GEFT,Rac/Cdc42/Rho exchange factor GEFT,RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT,p63RhoGEF
3	C, F (C, F)	Transforming protein RhoA	polymer	196	22132.5	2	UniProt (P61586) Pfam (PF00071)	Homo sapiens (Human)	Rho cDNA clone 12,h12
4	G, J (A, D)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	2	Chemie (ALF)
5	H, K (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	I, L (A, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
7	M, N (A, D)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, D: 7 - 34 (UniProt: P10824)

PDB	External Database	Details
Arg 35	-	linker
Ser 36	-	linker

A, D: 37 - 359 (PDB: 2RGN)
C, F: 1 - 193 (UniProt: P61586)

PDB	External Database	Details
Gly -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	208073.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1141.0
All*	Total formula weight	209214.5

*Water molecules are not included.