2RET

The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the Type 2 Secretion System of Vibrio vulnificus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Pseudopilin EpsI	polymer	103	11689.3	4	UniProt (Q7MPZ1) Pfam (PF02501)	Vibrio vulnificus
2	B, D, F, H (B, D, F, H)	EpsJ	polymer	175	20734.7	4	Pfam (PF11612) UniProt (by SIFTS) (Q7MPZ0)	Vibrio vulnificus
3	I, J (B, F)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	K, L (F, H)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	611	Chemie (HOH)

Sequence modifications

A, C, E, G: 25 - 110 (UniProt: Q7MPZ1)

PDB	External Database	Details
Mse 24	-	expression tag
Thr 96	Glu 128	engineered mutation
Thr 97	Lys 129	engineered mutation
Ser 111	-	expression tag
Glu 112	-	expression tag
Asn 113	-	expression tag
Leu 114	-	expression tag
Tyr 115	-	expression tag
Phe 116	-	expression tag
Gln 117	-	expression tag
Gly 118	-	expression tag
Gly 119	-	expression tag
Gly 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	129696.1
Non-Polymers*	Number of molecules	4
	Total formula weight	116.9
All*	Total formula weight	129812.9

*Water molecules are not included.