2RET

The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the Type 2 Secretion System of Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-29
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97903
Spacegroup name	P 1
Unit cell lengths	63.288, 79.145, 82.079
Unit cell angles	65.00, 69.19, 69.49

Refinement procedure

Resolution	40.390 - 2.210
R-factor	0.188
Rwork	0.185
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.457
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.085	0.063	0.501
Number of reflections	63933
<I/σ(I)>	10
Completeness [%]	97.5	98.6	91.8
Redundancy	3.8	3.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	298	10-15% PEG 4000 and 0.1 M MnCl2, protein buffer: 20 mM Tris-Hcl (pH 7.8), 250 mM NaCl, 1 mM EDTA, VAPOR DIFFUSION, SITTING DROP, temperature 298K