2R40

Crystal structure of 20E bound EcR/USP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Ecdysone Receptor	polymer	266	30369.0	1	UniProt (O18473) Pfam (PF00104)	Heliothis virescens (tobacco budworm)	Ecdysteroid receptor; 20-hydroxy-ecdysone receptor; 20E receptor; EcRH; HvEcR
2	B (A)	Ultraspiracle	polymer	263	29951.8	1	UniProt (Q7SIF6) Pfam (PF00104)	Heliothis virescens (tobacco budworm)	Gene regulation protein
3	C (D)	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
4	D (D)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	E (D)	(2beta,3beta,5beta,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one	non-polymer		480.6	1	Chemie (20E)
6	F (D)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	G (A)	L-ALPHA-PHOSPHATIDYL-BETA-OLEOYL-GAMMA-PALMITOYL-PHOSPHATIDYLETHANOLAMINE	non-polymer		709.9	1	Chemie (EPH)
8	H, I (D, A)	water	water		18.0	85	Chemie (HOH)

Sequence modifications

D: 284 - 532 (UniProt: O18473)

PDB	External Database	Details
Gly 267	-	expression tag
Ser 268	-	expression tag
His 269	-	expression tag
Met 270	-	expression tag
Ala 271	-	expression tag
Ser 272	-	expression tag
Thr 274	-	expression tag
Gly 275	-	expression tag
Gly 276	-	expression tag
Gln 277	-	expression tag
Met 279	-	expression tag
Gly 280	-	expression tag
Asp 282	-	expression tag
Pro 283	-	expression tag
Gln 321	-	SEE REMARK 999
Thr 322	-	SEE REMARK 999
Trp 323	-	SEE REMARK 999
Cys 483	Gly 501	SEE REMARK 999
Lys 489	Glu 507	SEE REMARK 999

A: 205 - 466 (UniProt: Q7SIF6)

PDB	External Database	Details
Met 204	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60320.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1567.8
All*	Total formula weight	61888.5

*Water molecules are not included.