2R09

Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cytohesin-3	polymer	347	40838.0	2	UniProt (O08967) Pfam (PF01369) Pfam (PF00169) In PDB	Mus musculus (house mouse)	PH, SEC7 and coiled-coil domain-containing protein 3, CLM3, SEC7 homolog C, mSec7-3, ARF nucleotide-binding site opener 3, Protein ARNO3, General receptor of phosphoinositides 1, Grp1
2	A, B	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
3	A, B	INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE	non-polymer		500.1	2	Chemie (4IP)
4	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
5	A	3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL	non-polymer		398.5	1	Chemie (PE5)
6		water	water		18.0	807	Chemie (HOH)

Sequence modifications

A, B: 63 - 399 (UniProt: O08967)

PDB	External Database	Details
Mse 53	-	expression tag
Gly 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
His 57	-	expression tag
His 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
Gly 61	-	expression tag
Ser 62	-	expression tag
Ala 68	Lys 68	engineered mutation
Tyr 260	His 260	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81676.0
Non-Polymers*	Number of molecules	11
	Total formula weight	2221.3
All*	Total formula weight	83897.3

*Water molecules are not included.