2R09
Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791, 0.97872, 1.1627 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.599, 94.611, 115.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.20558 |
Rwork | 0.204 |
R-free | 0.24106 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.074 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.950 |
Number of reflections | 72844 |
Completeness [%] | 99.9 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | 10-15% PEG 6000, 50 mM Tris, pH 8.0, 0.2 M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |