2QNC
Crystal structure of T4 Endonuclease VII N62D mutant in complex with a DNA Holliday junction
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA (5'-D(*DCP*DG*DAP*DAP*DGP*DAP*DAP*DTP*DTP*DCP*DCP*DGP*DGP*DAP*DTP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DCP*DC)-3') | polymer | 24 | 7418.8 | 1 | |||
| 2 | B (D) | DNA (5'-D(*DGP*DGP*DAP*DTP*DCP*DCP*DCP*DTP*DAP*DAP*DGP*DCP*DTP*DCP*DCP*DAP*DTP*DCP*DGP*DAP*DTP*DGP*DTP*DG)-3') | polymer | 24 | 7345.7 | 1 | |||
| 3 | C (E) | DNA (5'-D(*DCP*DAP*DCP*DAP*DTP*DCP*DGP*DAP*DTP*DGP*DGP*DAP*DGP*DCP*DCP*DGP*DCP*DTP*DAP*DGP*DGP*DCP*DCP*DT)-3') | polymer | 24 | 7355.7 | 1 | |||
| 4 | D (F) | DNA (5'-D(*DAP*DGP*DGP*DCP*DCP*DTP*DAP*DGP*DCP*DGP*DTP*DCP*DCP*DGP*DGP*DAP*DAP*DTP*DTP*DCP*DTP*DTP*DCP*DG)-3') | polymer | 24 | 7361.7 | 1 | |||
| 5 | E, F (A, B) | Recombination endonuclease VII | polymer | 157 | 18174.8 | 2 | UniProt (P13340) Pfam (PF02945) Pfam (PF09124) | Enterobacteria phage T4 | Endo VII, Protein Gp49 |
| 6 | G, I (F, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | H, J (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 8 | K, L (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 9 | M, N, O, P (D, E, A, B) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 157 (UniProt: P13340)
| PDB | External Database | Details |
|---|---|---|
| Asp 62 | Asn 62 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 65831.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 303.6 | |
| All* | Total formula weight | 66135.1 |
