2QKK
Human RNase H catalytic domain mutant D210N in complex with 14-mer RNA/DNA hybrid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | C, G, K, O, T... | 5'-R(*CP*GP*AP*CP*AP*CP*CP*UP*GP*AP*UP*UP*CP*C)-3' | polymer | 14 | 4382.7 | 6 | |||
| 2 | D, H, L, P, U... | 5'-D(*GP*GP*AP*AP*TP*CP*AP*GP*GP*TP*GP*TP*CP*G)-3' | polymer | 14 | 4360.8 | 6 | |||
| 3 | A, B, E, F, I... | Ribonuclease H1 | polymer | 154 | 17104.3 | 11 | UniProt (O60930) Pfam (PF00075) In PDB | Homo sapiens (human) | Hs-RNase HC; RNase H1; Ribonuclease H type II |
| 4 | D | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 5 | B, E, F, I, J... | CALCIUM ION | non-polymer | 40.1 | 14 | Chemie (CA) | |||
| 6 | A | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 7 | B | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 8 | water | water | 18.0 | 172 | Chemie (HOH) |
Sequence modifications
A, B, E, F, I, J, M, N, R, S, W: 136 - 286 (UniProt: O60930)
| PDB | External Database | Details |
|---|---|---|
| Gly 133 | - | expression tag |
| Ser 134 | - | expression tag |
| His 135 | - | expression tag |
| Asn 210 | Asp 210 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 23 |
| Total formula weight | 240608.1 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 913.9 | |
| All* | Total formula weight | 241522.0 |
