2QKB

Human RNase H catalytic domain mutant D210N in complex with 20-mer RNA/DNA hybrid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	5'-R(*GP*GP*AP*GP*UP*GP*CP*GP*AP*CP*AP*CP*CP*UP*GP*AP*UP*UP*CP*C)-3')	polymer	20	6382.9	1
2	B (D)	5'-D(*DGP*DGP*DAP*DAP*DTP*DCP*DAP*DGP*DGP*DTP*DGP*DTP*DCP*DGP*DCP*DAP*DCP*DTP*DCP*DT)-3'	polymer	20	6150.0	1
3	C, D (A, B)	Ribonuclease H1	polymer	154	17291.9	2	UniProt (O60930) Pfam (PF00075)	Homo sapiens (human)	Hs-RNase HC; RNase H1; Ribonuclease H type II
4	E, G (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	F, H (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	I, J, K, L (C, D, A, B)	water	water		18.0	58	Chemie (HOH)

Sequence modifications

A, B: 136 - 286 (UniProt: O60930)

PDB	External Database	Details
Gly 133	-	expression tag
Ser 134	-	expression tag
His 135	-	expression tag
Asn 210	Asp 210	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47116.6
Non-Polymers*	Number of molecules	4
	Total formula weight	316.3
All*	Total formula weight	47432.8

*Water molecules are not included.