2QCS
A complex structure between the Catalytic and Regulatory subunit of Protein Kinase A that represents the inhibited state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | cAMP-dependent protein kinase, alpha-catalytic subunit | polymer | 350 | 40737.3 | 1 | UniProt (P05132) Pfam (PF00069) | Mus musculus (house mouse) | PKA C-alpha |
| 2 | B (B) | cAMP-dependent protein kinase type I-alpha regulatory subunit | polymer | 291 | 32723.1 | 1 | UniProt (P00514) Pfam (PF00027) | Bos taurus (cattle) | |
| 3 | C, D (A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 4 | E, K, L, M, N... (A, B) | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
| 5 | F (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 6 | G (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 7 | H (A) | TRIS(HYDROXYETHYL)AMINOMETHANE | non-polymer | 163.2 | 1 | Chemie (TAM) | |||
| 8 | I, J, P (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 9 | Q, R (A, B) | water | water | 18.0 | 289 | Chemie (HOH) |
Sequence modifications
A: 1 - 350 (UniProt: P05132)
B: 90 - 379 (UniProt: P00514)
| PDB | External Database | Details |
|---|---|---|
| Sep 139 | Ser 140 | modified residue |
| Tpo 197 | Thr 198 | modified residue |
| Sep 338 | Ser 339 | modified residue |
| PDB | External Database | Details |
|---|---|---|
| Lys 333 | Arg 334 | engineered mutation |
| Ala 380 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 73460.4 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1691.0 | |
| All* | Total formula weight | 75151.4 |
