2Q57

X-ray structure of Cerulean GFP: A tryptophan-based chromophore useful for fluorescence lifetime imaging

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cerulean Green fluorescent protein	polymer	256	28703.4	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria
2		water	water		18.0	149	Chemie (HOH)

Sequence modifications

A: 1 - 238 (UniProt: P42212)

PDB	External Database	Details
Met -17	-	expression tag
Arg -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Ala -5	-	expression tag
Leu -4	-	expression tag
Pro -3	-	expression tag
Val -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag
Leu 64	Phe 64	engineered mutation
Crf 66	Ser 65	chromophore
Crf 66	Tyr 66	chromophore
Crf 66	Gly 67	chromophore
Ala 72	Ser 72	engineered mutation
Arg 80	Gln 80	engineered mutation
Ala 145	Tyr 145	engineered mutation
Ile 146	Asn 146	engineered mutation
Asp 148	His 148	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Leu 231	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28703.4
All*	Total formula weight	28703.4

*Water molecules are not included.