2Q57
X-ray structure of Cerulean GFP: A tryptophan-based chromophore useful for fluorescence lifetime imaging
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.232, 63.339, 69.821 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.000 |
| R-factor | 0.19172 |
| Rwork | 0.188 |
| R-free | 0.26206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oxd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.866 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.207 |
| Number of reflections | 15852 | |
| <I/σ(I)> | 5.1 | 1.8 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100mM sodium acetate, 19% PEG 4000, 1mM EDTA, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
