2PYJ
Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, F (X, Q, J) | 5'-d(GACTGCTTA(DOC)-3' | polymer | 10 | 3004.0 | 3 | |||
| 2 | B, D, E (Y, R, K) | 5'-d(ACACGTAAGCAGTC)-3' | polymer | 14 | 4273.8 | 3 | |||
| 3 | G, H (A, B) | DNA polymerase | polymer | 575 | 66720.9 | 2 | UniProt (P03680) Pfam (PF03175) | Bacillus phage phi29 | Early protein GP2 |
| 4 | AA, AB, BA, BB, CA... (A, B, X, Y, Q...) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 41 | Chemie (EDO) | |||
| 5 | LA, R (B, A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 6 | MA, S (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | NA, T (B, A) | 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (DGT) | |||
| 8 | DB, EB, FB, GB, HB... (X, Y, Q, R, K...) | water | water | 18.0 | 969 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 575 (UniProt: P03680)
| PDB | External Database | Details |
|---|---|---|
| Ala 12 | Asp 12 | engineered mutation |
| Ala 66 | Asp 66 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 155275.2 | |
| Non-Polymers* | Number of molecules | 47 |
| Total formula weight | 3717.6 | |
| All* | Total formula weight | 158992.9 |
