2PYJ
Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.835, 114.667, 104.761 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 41.070 - 2.030 |
| R-factor | 0.19128 |
| Rwork | 0.189 |
| R-free | 0.23409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | copy c of 1xhx without residues 359-394 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.076 | 0.353 |
| Number of reflections | 107450 | |
| <I/σ(I)> | 16.3 | 2.4 |
| Completeness [%] | 98.0 | 83.9 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM sodium acetate, 200 mM ammonium acetate, 15% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | sodium acetate | ||
| 5 | 1 | 2 | ammonium acetate | ||
| 6 | 1 | 2 | PEG 4000 |
