2PFO
DNA Polymerase lambda in complex with DNA and dUPNPP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | Template | polymer | 11 | 3374.2 | 1 | |||
| 2 | B (P) | Primer | polymer | 6 | 1793.2 | 1 | |||
| 3 | C (D) | Downstream Primer | polymer | 4 | 1191.8 | 1 | |||
| 4 | D (A) | DNA polymerase lambda | polymer | 335 | 37447.7 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (human) | Pol Lambda, DNA polymerase kappa, DNA polymerase beta-2, Pol beta2 |
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 7 | G, H, I, J (A) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 8 | K (A) | 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DUP) | |||
| 9 | L (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 10 | M, N, O, P (T, P, D, A) | water | water | 18.0 | 329 | Chemie (HOH) |
Sequence modifications
A: 242 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Met 241 | - | initiating methionine |
| Ala 543 | Cys 543 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 43806.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 700.4 | |
| All* | Total formula weight | 44507.4 |
