2P9E

Crystal Structure of G336V mutant of E.coli phosphoglycerate dehydrogenase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	D-3-phosphoglycerate dehydrogenase	polymer	410	44140.4	4	UniProt (P0A9T0) Pfam (PF00389) Pfam (PF02826) Pfam (PF22629)	Escherichia coli	PGDH
2	E, F, J, K, N... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4) PubChem (1061)
3	G, L, Q, V (A, B, C, D)	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	4	Chemie (NAI) PubChem (16219774) PubChem (928) PubChem (44119956) PubChem (44415182) PubChem (439153) PubChem (124934832) PubChem (126455899) PubChem (94865921) PubChem (94865923) PubChem (134693949) PubChem (12309339) PubChem (12309347) PubChem (124432988) PubChem (140875346) PubChem (11250888) PubChem (12309341) PubChem (124934831) PubChem (124934833) PubChem (162951601) PubChem (162951600) PubChem (164575491) PubChem (169545638) PubChem (170905224) PubChem (174316169) PubChem (129607000) PubChem (129628338) PubChem (146167236) PubChem (162951602) PubChem (174084875)
4	H, I, M, R, S... (A, B, C, D)	CITRIC ACID	non-polymer		192.1	6	Chemie (CIT) PubChem (311)
5	P (C)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	AA, X, Y, Z (D, A, B, C)	water	water		18.0	82	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 410 (UniProt: P0A9T0)

PDB	External Database	Details
Ala 81	Cys 81	engineered mutation
Ala 83	Cys 83	engineered mutation
Ala 250	Cys 250	engineered mutation
Ala 282	Cys 282	engineered mutation
Val 336	Gly 336	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	176561.8
Non-Polymers*	Number of molecules	19
	Total formula weight	4670.3
All*	Total formula weight	181232.1

*Water molecules are not included.