2P9E
Crystal Structure of G336V mutant of E.coli phosphoglycerate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 |
| Unit cell lengths | 76.237, 76.237, 353.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.600 |
| R-factor | 0.2098 |
| Rwork | 0.206 |
| R-free | 0.27923 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pa3 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.165 | 0.846 |
| Number of reflections | 61423 | |
| <I/σ(I)> | 19.3 | 1.64 |
| Completeness [%] | 99.5 | 96.1 |
| Redundancy | 18.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1.5M AmSO4, 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
