2OZL

Human pyruvate dehydrogenase S264E variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Pyruvate dehydrogenase E1 component alpha subunit, somatic form	polymer	365	40756.5	2	UniProt (P08559) Pfam (PF00676) In PDB	Homo sapiens (human)	PDHE1-A type I
2	B, D	Pyruvate dehydrogenase E1 component subunit beta	polymer	341	37346.8	2	UniProt (P11177) Pfam (PF02779) Pfam (PF02780) In PDB	Homo sapiens (human)	PDHE1-B
3	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, C	THIAMINE DIPHOSPHATE	non-polymer		425.3	2	Chemie (TPP)
5	B, D	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
6		water	water		18.0	757	Chemie (HOH)

Sequence modifications

A, C: 1 - 361 (UniProt: P08559)

PDB	External Database	Details
Met -3	-	cloning artifact
Arg -2	-	cloning artifact
Gly -1	-	cloning artifact
Ser 0	29	cloning artifact
Glu 264	Ser 293	engineered mutation

B, D: 2 - 329 (UniProt: P11177)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	30	expression tag
Leu 1	31	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156206.7
Non-Polymers*	Number of molecules	6
	Total formula weight	977.4
All*	Total formula weight	157184.2

*Water molecules are not included.