2OZL
Human pyruvate dehydrogenase S264E variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Pyruvate dehydrogenase E1 component alpha subunit, somatic form | polymer | 365 | 40756.5 | 2 | UniProt (P08559) Pfam (PF00676) | Homo sapiens (human) | PDHE1-A type I |
| 2 | B, D (B, D) | Pyruvate dehydrogenase E1 component subunit beta | polymer | 341 | 37346.8 | 2 | UniProt (P11177) Pfam (PF02779) Pfam (PF02780) | Homo sapiens (human) | PDHE1-B |
| 3 | E, H (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | F, I (A, C) | THIAMINE DIPHOSPHATE | non-polymer | 425.3 | 2 | Chemie (TPP) | |||
| 5 | G, J (B, D) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 6 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 757 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 361 (UniProt: P08559)
B, D: 2 - 329 (UniProt: P11177)
| PDB | External Database | Details |
|---|---|---|
| Met -3 | - | cloning artifact |
| Arg -2 | - | cloning artifact |
| Gly -1 | - | cloning artifact |
| Ser 0 | 29 | cloning artifact |
| Glu 264 | Ser 293 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -11 | - | expression tag |
| Arg -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Ser -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | 30 | expression tag |
| Leu 1 | 31 | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 156206.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 977.4 | |
| All* | Total formula weight | 157184.2 |
