2OTE

Crystal structure of a monomeric cyan fluorescent protein in the photobleached state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	GFP-like fluorescent chromoprotein cFP484	polymer	216	24799.2	2	UniProt (Q9U6Y3) Pfam (PF01353) In PDB	Clavularia sp.
2	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
3		water	water		18.0	450	Chemie (HOH)

Sequence modifications

A, B: 6 - 221 (UniProt: Q9U6Y3)

PDB	External Database	Details
Asn 42	His 80	engineered mutation
Ile 44	Leu 82	engineered mutation
Thr 62	Ser 100	engineered mutation
Pia 66	Gln 104	chromophore
Pia 66	Tyr 105	chromophore
Pia 66	Gly 106	chromophore
Phe 72	Leu 110	engineered mutation
Pro 80	Ala 118	engineered mutation
Asn 81	Asp 119	engineered mutation
His 123	Arg 161	engineered mutation
Leu 124	Phe 162	engineered mutation
Lys 125	Asp 163	engineered mutation
Glu 127	Met 165	engineered mutation
Leu 150	Met 188	engineered mutation
Lys 162	Ser 200	engineered mutation
Lys 164	Ser 202	engineered mutation
His 173	Tyr 211	engineered mutation
Val 175	Cys 213	engineered mutation
Thr 179	Ser 217	engineered mutation
Arg 182	Lys 220	engineered mutation
Ala 186	Val 224	engineered mutation
Val 213	Leu 251	engineered mutation
Ser 216	Asn 254	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49598.4
Non-Polymers*	Number of molecules	1
	Total formula weight	59.0
All*	Total formula weight	49657.4

*Water molecules are not included.