2OTE
Crystal structure of a monomeric cyan fluorescent protein in the photobleached state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.489, 66.310, 97.203 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.470 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2OTB with the waters and chromophores removed. |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.054 | 0.449 |
| Number of reflections | 68960 | |
| <I/σ(I)> | 16.4 | 2.4 |
| Completeness [%] | 94.7 | 91.2 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 31% PEG 3400, 0.2 M Lithium Sulfate, 0.1 M Sodium Acetate, 2.9 mM 1-s-Nonyl-beta-D-thioglucoside, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
