2OIN

crystal structure of HCV NS3-4A R155K mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Polyprotein	polymer	200	21205.2	2	UniProt (Q9ELS8) Pfam (PF02907) UniProt (by SIFTS) (P27958)	Hepatitis C virus
2	C, D (C, D)	NS4A peptide	polymer	21	2077.6	2	Pfam (PF01006) UniProt (by SIFTS) (P27958)	synthetic construct
3	E, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	G, H, I, J (A, B, C, D)	water	water		18.0	206	Chemie (HOH)

Sequence modifications

A, B: 27 - 207 (UniProt: Q9ELS8)

PDB	External Database	Details
Met 16	-	cloning artifact
Ala 17	-	cloning artifact
Ser 18	-	cloning artifact
Met 19	-	cloning artifact
Thr 20	-	cloning artifact
Gly 21	-	cloning artifact
Gly 22	-	cloning artifact
Gln 23	-	cloning artifact
Gln 24	-	cloning artifact
Met 25	-	cloning artifact
Gly 26	-	cloning artifact
Arg 145	Gln 1145	conflict
Lys 181	Arg 1181	engineered mutation
Gly 208	-	cloning artifact
Ser 209	-	cloning artifact
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	46565.6
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	46696.4

*Water molecules are not included.