2OD9
Structural Basis for Nicotinamide Inhibition and Base Exchange in Sir2 Enzymes
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | NAD-dependent deacetylase HST2 | polymer | 308 | 34794.8 | 1 | UniProt (P53686) Pfam (PF02146) | Saccharomyces cerevisiae (baker's yeast) | Homologous to SIR2 protein 2 |
| 2 | B (B) | H4 peptide | polymer | 14 | 1610.9 | 1 | |||
| 3 | C (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 4 | D (A) | 5'-O-[(S)-{[(S)-{[(2R,3R,4S)-3,4-DIHYDROXYPYRROLIDIN-2-YL]METHOXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]ADENOSINE | non-polymer | 542.3 | 1 | Chemie (A1R) | |||
| 5 | E (A) | NICOTINAMIDE | non-polymer | 122.1 | 1 | Chemie (NCA) | |||
| 6 | F, G (A, B) | water | water | 18.0 | 116 | Chemie (HOH) |
Sequence modifications
A: 1 - 294 (UniProt: P53686)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Arg -12 | - | cloning artifact |
| Gly -11 | - | cloning artifact |
| Ser -10 | - | cloning artifact |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | cloning artifact |
| Met -2 | - | cloning artifact |
| Ala -1 | - | cloning artifact |
| Ser 0 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 36405.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 729.9 | |
| All* | Total formula weight | 37135.6 |
