2O6V

Crystal structure and solution NMR studies of Lys48-linked tetraubiquitin at neutral pH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Ubiquitin	polymer	76	8576.8	4	UniProt (P62988) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens (human)
2	B, F (B, F)	Ubiquitin	polymer	76	8621.9	2	UniProt (P62988) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens (human)
3	D, H (D, H)	Ubiquitin	polymer	76	8632.9	2	UniProt (P62988) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens (human)
4	I, J, L, N (A, B, D, E)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5	K, M (B, D)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
6	O, P, Q, R, S... (A, B, C, D, E...)	water	water		18.0	149	Chemie (HOH)

Sequence modifications

B, F: 101 - 176 (UniProt: P62988)

PDB	External Database	Details
Slz 148	Lys 48	engineered mutation
Arg 163	Lys 63	engineered mutation

D, H: 301 - 376 (UniProt: P62988)

PDB	External Database	Details
Arg 348	Lys 48	engineered mutation
Arg 363	Lys 63	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	68816.8
Non-Polymers*	Number of molecules	6
	Total formula weight	774.7
All*	Total formula weight	69591.5

*Water molecules are not included.