2O6V

Crystal structure and solution NMR studies of Lys48-linked tetraubiquitin at neutral pH

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 801

Chain	Residue
A	ARG42
A	GLN49
A	ARG72
B	ARG142
B	GLN149
B	ARG172

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 802

Chain	Residue
C	ARG254
B	GLY110
B	LYS111
B	THR112

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 803

Chain	Residue
A	ARG54
D	THR312

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 E 804

Chain	Residue
E	ARG442
E	GLN449
E	ARG472
F	ARG542
F	GLN549
F	ARG572

---

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MES B 901

Chain	Residue
B	LYS129

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MES D 902

Chain	Residue
D	PHE304
D	LYS306
D	THR366
D	HIS368

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGrqleD

Chain	Residue	Details
D	LYS327-ASP352
B	LYS127-ASP152
A	LYS27-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
D	ARG354
D	ARG372
H	ARG754
H	ARG772
E	ARG454
E	ARG472
G	ARG654
G	ARG672

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Essential for function

Chain	Residue	Details
D	HIS368
H	HIS768
E	HIS468
G	HIS668

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
D	SER365
H	SER765
E	SER465
G	SER665

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
D	THR366
H	THR766
E	THR466
G	THR666

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742

Chain	Residue	Details
D	GLY376
H	GLY776
E	GLY476
G	GLY676

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
D	LYS306
H	LYS706
E	LYS406
G	LYS606

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
D	GLY376
H	GLY776
E	GLY476
G	GLY676

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
D	LYS311
D	ARG348
H	LYS711
H	ARG748
E	LYS411
E	LYS448
G	LYS611
G	LYS648

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
D	LYS327
H	LYS727
E	LYS427
G	LYS627

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
D	LYS329
H	LYS729
E	LYS429
G	LYS629

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
D	LYS333
H	LYS733
E	LYS433
G	LYS633

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
D	ARG363
H	ARG763
E	LYS463
G	LYS663

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