2O5J

Crystal structure of the T. thermophilus RNAP polymerase elongation complex with the NTP substrate analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (G, X)	5'-D(P*CP*CP*CP*TP*GP*TP*CP*TP*GP*GP*CP*GP*TP*TP*CP*GP*CP*GP*CP*GP*CP*CP*G)-3'	polymer	23	7001.5	2
2	B, E (H, Y)	5'-R(P*GP*AP*GP*UP*CP*UP*GP*CP*GP*GP*CP*GP*CP*GP*CP*G)-3'	polymer	16	5184.1	2
3	C, F (I, Z)	5'-D(*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*AP*GP*GP*G)-3'	polymer	14	4323.8	2
4	G, H, L, M (A, B, K, L)	DNA-directed RNA polymerase alpha chain	polymer	315	35056.2	4	UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Thermus thermophilus	RNAP alpha subunit, Transcriptase alpha chain, RNA polymerase subunit alpha
5	I, N (C, M)	DNA-directed RNA polymerase beta chain	polymer	1119	125436.5	2	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Thermus thermophilus	RNAP beta subunit, Transcriptase beta chain, RNA polymerase subunit beta
6	J, O (D, N)	DNA-directed RNA polymerase beta' chain	polymer	1524	170997.4	2	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Thermus thermophilus	RNAP beta' subunit, Transcriptase beta' chain, RNA polymerase beta' subunit
7	K, P (E, O)	DNA-directed RNA polymerase omega chain	polymer	99	11533.3	2	UniProt (Q8RQE7) Pfam (PF01192)	Thermus thermophilus	RNAP omega subunit, Transcriptase omega chain, RNA polymerase omega subunit
8	Q, R, V, W (D, N)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
9	S, T, X, Y (D, N)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
10	U, Z (D, N)	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	2	Chemie (APC)
11	AA, BA, CA, DA, EA... (G, H, I, X, Y...)	water	water		18.0	2633	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	789178.0
Non-Polymers*	Number of molecules	10
	Total formula weight	1369.3
All*	Total formula weight	790547.3

*Water molecules are not included.