2O5J
Crystal structure of the T. thermophilus RNAP polymerase elongation complex with the NTP substrate analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 152.340, 152.340, 524.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.2293 |
| Rwork | 0.225 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5i |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.820 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.077 | 0.441 |
| Number of reflections | 211633 | |
| <I/σ(I)> | 11.7 | 2.1 |
| Completeness [%] | 89.3 | 75.5 |
| Redundancy | 3.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% MPD, 0.1M Na cacodylate, 0.2M Mg acetate, 2mM AMPcPP, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Na cacodylate | ||
| 3 | 1 | 1 | Mg acetate | ||
| 4 | 1 | 1 | AMPcPP | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | Na cacodylate | ||
| 8 | 1 | 2 | Mg acetate | ||
| 9 | 1 | 2 | H2O |
