2O26

Structure of a class III RTK signaling assembly

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, E, F)	Kit ligand	polymer	145	16444.8	4	UniProt (P20826) Pfam (PF02404)	Mus musculus (house mouse)	C-kit ligand, Stem cell factor, SCF, Mast cell growth factor, MGF, Hematopoietic growth factor KL, Steel factor
2	E, F, G, H (X, Y, U, W)	Mast/stem cell growth factor receptor	polymer	290	32274.6	4	UniProt (P05532) Pfam (PF00047)	Mus musculus (house mouse)	SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen
3	I, K, M, O (C, G, I, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	4	In PDB GlyTouCan (G28454KX)
4	J, L, N, P (D, H, J, L)	alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G62182OO)
5	Q, R, S, T, U... (A, B, E, F, X...)	water	water		18.0	1730	Chemie (HOH)

Sequence modifications

A, B, E, F: 3 - 141 (UniProt: P20826)

PDB	External Database	Details
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag

X, Y, U, W: 25 - 314 (UniProt: P05532)

PDB	External Database	Details
Gln 146	Asn 146	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	194877.3
Branched	Number of molecules	8
	Total formula weight	4628.3
All*	Total formula weight	199505.7

*Water molecules are not included.