2O26
Structure of a class III RTK signaling assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.849, 200.154, 82.023 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.500 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.27000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.040 | 0.389 |
| Number of reflections | 82592 | |
| <I/σ(I)> | 17.1 | 2.6 |
| Completeness [%] | 96.8 | 93.3 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
