2NX3
Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | 2-dehydro-3-deoxyphosphooctonate aldolase | polymer | 267 | 29738.4 | 12 | UniProt (O66496) Pfam (PF00793) | Aquifex aeolicus | Phospho-2-dehydro-3-deoxyoctonate aldolase, 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase, KDO-8-phosphate synthetase, KDO 8-P synthase, KDOPS |
| 2 | BA, DA, FA, HA, M... (I, J, K, L, A...) | PHOSPHOENOLPYRUVATE | non-polymer | 168.0 | 11 | Chemie (PEP) | |||
| 3 | AA, CA, EA, GA, IA... (H, I, J, K, L...) | ARABINOSE-5-PHOSPHATE | non-polymer | 232.1 | 11 | Chemie (A5P) | |||
| 4 | S (D) | (2R,4R,5R,6R,7R)-2,4,5,6,7-PENTAHYDROXY-2,8-BIS(PHOSPHONOOXY)OCTANOIC ACID | non-polymer | 416.2 | 1 | Chemie (1NT) | |||
| 5 | JA, KA, LA, MA, NA... (A, B, C, D, E...) | water | water | 18.0 | 1624 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H, I, J, K, L: 1 - 267 (UniProt: O66496)
| PDB | External Database | Details |
|---|---|---|
| Asn 11 | Cys 11 | engineered mutation |
| Pro 235 | Ser 235 | engineered mutation |
| Ala 237 | Gln 237 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 356860.3 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 4818.0 | |
| All* | Total formula weight | 361678.3 |
